Chinese translation for "dna ligase"
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- dna连接酶,多脱氧核糖核苷酸合酶
dna黏合酶
脱氧核糖核酸连接酶
Related Translations:
dna电泳: dna electrophoresis
- Example Sentences:
| 1. | Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。 | | 2. | 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒，用kpni和bamhi酶切，分别回收cpti片断和酶切后的载体片段，用t _ 4连接酶连接构建成中间载体pgem - cp 。 | | 3. | Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli
Hbsag质粒与ppiczaa载体分别经xhol和xbaln切，再在t4dna连接酶作用下进行连接，获得工程菌表达型ppiczaas ； hbsag质粒，转化大肠杆菌t0p10细胞，经xhol和xbal与sacll和xbal酶切电泳，证实s ； 。 | | 4. | At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0软件，对所克隆的序列用相邻接点法( neighborjoining州j ） method ）进行多序列比对，分析其同源性，并构建基因进化树。 | | 5. | The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理， t _ 4dna连接酶连接，用连接产物转化大肠杆菌jm109感受态细胞，得到重组转移载体质粒pfastbac - gedna 。 | | 6. | A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接；用连接产物转化大肠杆菌jml09感受态细胞，置含amp 、 x - gal和iptg的lb平板上培养12 20小时；挑取白色菌落于选择性培养基扩大培养，碱裂解法小量提取质粒dna ，并进行酶切分析鉴定，结果获得整合有prvge基因的重组质粒pugedna ，并与其它prv分离株进行ge基因序列同源性分析。 | | 7. | First , the purified pezzis and pcr product of angiostatin are digested by ecor . i and xba i . after purifying the digested products respectively , we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as . then transform it to the competent e . coli dh5a
用限制性内切酶ecori与xbai对目的基因as 、表达载体pezz18行双酶切，酶切产物纯化后利用大肠杆菌t _ 4dna连接酶连接构成重组子pezz18 - as ，并转化e . colidh5 ，经氨苄青霉素lb平板初筛后，以菌液pcr和重组子的单、双酶切行进一步鉴定。 |
- Similar Words：
- "dna jumping technique" Chinese translation, "dna lab" Chinese translation, "dna lac operon" Chinese translation, "dna ladder" Chinese translation, "dna library" Chinese translation, "dna ligasedna" Chinese translation, "dna linear energy transfer" Chinese translation, "dna marker" Chinese translation, "dna mediated gene transfer" Chinese translation, "dna melting" Chinese translation
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